Review



incucyte system  (Sartorius AG)


Bioz Verified Symbol Sartorius AG is a verified supplier
Bioz Manufacturer Symbol Sartorius AG manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Sartorius AG incucyte system
    Incucyte System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 16012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pm42826203-308-9-11
    Average 99 stars, based on 16012 article reviews
    incucyte system - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Titration:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Sensitivity:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Virus:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Analysis:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Neutralization:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Software:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Concentration Assay:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Incubation:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Antibody Labeling:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Labeling:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Cell Culture:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Magnetic Cell Separation:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Transduction:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Selection:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Activity Assay:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir

    Microscopy:

    Article Title: Leishmania and phlebovirus seroprevalence in dogs in two non-endemic central European countries: Austria and Germany
    Article Snippet: included TCID 50 assay for viral titration confirmation. After 5 days for TOSV and 6 days for SFSV, photos of each well of the 96-well microplates were taken by using Incucyte SX5 Live-Cell Analysis (Sartorius, Göttingen, Germany) and results were analyzed to determine cytopathic effect (CPE) and neutralization titers. A sensitivity of 98.1% and specificity of 98.8% is calculated for CPE-based vir



    Similar Products

    99
    Sartorius AG incucyte system
    Incucyte System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pm42826203-308-9-11
    Average 99 stars, based on 1 article reviews
    incucyte system - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG incucyte live cell imaging system
    ART6043 potentiates PARP inhibition in vitro . A, Representative CTG assay combination matrix for DLD-1 BRCA2 −/− (left) and DLD-1 wild-type (right) cells treated with titrated ART6043 and talazoparib for 14 days. Intensity of blue color indicates the level of synergy. % Control indicates relative viability. B, Representative <t>IncuCyte</t> growth curves for DLD-1 BRCA2 −/− cells treated with ART6043 or talazoparib as monotherapy or in combination at the indicated doses for 14 days. C, As for A but using olaparib instead of talazoparib. D, As for B but using olaparib instead of talazoparib. ART6043 concentrations represent free drug in tissue culture medium.
    Incucyte Live Cell Imaging System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pmc13628093-108-5-9
    Average 99 stars, based on 1 article reviews
    incucyte live cell imaging system - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG 96 well plate nunc
    ART6043 potentiates PARP inhibition in vitro . A, Representative CTG assay combination matrix for DLD-1 BRCA2 −/− (left) and DLD-1 wild-type (right) cells treated with titrated ART6043 and talazoparib for 14 days. Intensity of blue color indicates the level of synergy. % Control indicates relative viability. B, Representative <t>IncuCyte</t> growth curves for DLD-1 BRCA2 −/− cells treated with ART6043 or talazoparib as monotherapy or in combination at the indicated doses for 14 days. C, As for A but using olaparib instead of talazoparib. D, As for B but using olaparib instead of talazoparib. ART6043 concentrations represent free drug in tissue culture medium.
    96 Well Plate Nunc, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pm42821519-125-19-35
    Average 99 stars, based on 1 article reviews
    96 well plate nunc - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG incucyte s3 imager
    CLSP-1025 demonstrates functional activity in various cellular systems including cell cocultures and patient-derived organoids. A, CLSP-1025–mediated cytotoxicity in cocultures of PBMCs and HLA-A*02:01 + –p53 R175H+ tumor cells. Cytotoxicity is reported as the percent tumor-cell death after a 96-hour coculture (mean ± SEM; n = 2). B, HLA-A*02:01 surface expression on p53 R175H+ TYK-nu cells after 72 hours of IFN-γ treatment, measured by geometric mean fluorescence intensity (gMFI) via flow cytometry. Data shown as mean; n = 3 technical replicates. C, CLSP-1025–mediated cytotoxicity in PBMC cocultures with TYK-nu cells with or without IFN-γ pretreatment, shown as percent tumor-cell death (mean ± SEM; n = 3 technical replicates). D–E, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 R175H + adenocarcinoma organoids, measured by percent tumor-cell death and MSD, respectively [mean ± standard deviation (SD); n = 1 PBMC donor and technical duplicates for IFN-γ measurements]. F and G, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 WT adenocarcinoma organoids. H, CLSP-1025–mediated killing of GFP + KLE cells when cocultured with isolated CD4 + , CD8 + , or bulk CD3 + T cells. GFP signal was quantified in real time using <t>Incucyte</t> and plotted as normalized fluorescence units [GFP fluorescence units (GFU) × μm 2 /image]. Data shown as mean ± SD; n = 2 technical replicates.
    Incucyte S3 Imager, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pmc13628097-77-6-9
    Average 99 stars, based on 1 article reviews
    incucyte s3 imager - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG incucyte zoom
    CLSP-1025 demonstrates functional activity in various cellular systems including cell cocultures and patient-derived organoids. A, CLSP-1025–mediated cytotoxicity in cocultures of PBMCs and HLA-A*02:01 + –p53 R175H+ tumor cells. Cytotoxicity is reported as the percent tumor-cell death after a 96-hour coculture (mean ± SEM; n = 2). B, HLA-A*02:01 surface expression on p53 R175H+ TYK-nu cells after 72 hours of IFN-γ treatment, measured by geometric mean fluorescence intensity (gMFI) via flow cytometry. Data shown as mean; n = 3 technical replicates. C, CLSP-1025–mediated cytotoxicity in PBMC cocultures with TYK-nu cells with or without IFN-γ pretreatment, shown as percent tumor-cell death (mean ± SEM; n = 3 technical replicates). D–E, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 R175H + adenocarcinoma organoids, measured by percent tumor-cell death and MSD, respectively [mean ± standard deviation (SD); n = 1 PBMC donor and technical duplicates for IFN-γ measurements]. F and G, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 WT adenocarcinoma organoids. H, CLSP-1025–mediated killing of GFP + KLE cells when cocultured with isolated CD4 + , CD8 + , or bulk CD3 + T cells. GFP signal was quantified in real time using <t>Incucyte</t> and plotted as normalized fluorescence units [GFP fluorescence units (GFU) × μm 2 /image]. Data shown as mean ± SD; n = 2 technical replicates.
    Incucyte Zoom, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pmc13625922-320-6-11
    Average 99 stars, based on 1 article reviews
    incucyte zoom - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG integrated gfp fluorescence
    Medium-dependent effects on RSV G protein size, relative protein signals in virus stocks, and viral spread. ( A ) Western blot analysis of soluble G (sG) protein derived from HEK293T cells (top), HEK293T GALE/GALK1 knockout cells (middle), and whole-cell lysates of RSV A-0594-EGFP-infected A549 cells (bottom, 24 hpi), all produced or infected in cell culture medium A, B or C. ( B ) Culture medium-dependent viral spread of RSV A-0594-EGFP in A549 cells infected at an MOI of 1 and cultured in media A, B, or C. <t>GFP</t> integrated <t>fluorescence</t> intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system (Sartorius). Statistical significance was assessed by two-way ANOVA. ( C ) Virus spread following infection with RSV virus stocks A-C in A549 cells infected at an MOI of 1, with medium replaced by Opti-MEM (medium B) after adsorption. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system. ( D ) Relative G protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( E ) Relative F protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant
    Integrated Gfp Fluorescence, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pmc13621597-116-0-16
    Average 99 stars, based on 1 article reviews
    integrated gfp fluorescence - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG incucyte live cell analysis system
    Medium-dependent effects on RSV G protein size, relative protein signals in virus stocks, and viral spread. ( A ) Western blot analysis of soluble G (sG) protein derived from HEK293T cells (top), HEK293T GALE/GALK1 knockout cells (middle), and whole-cell lysates of RSV A-0594-EGFP-infected A549 cells (bottom, 24 hpi), all produced or infected in cell culture medium A, B or C. ( B ) Culture medium-dependent viral spread of RSV A-0594-EGFP in A549 cells infected at an MOI of 1 and cultured in media A, B, or C. <t>GFP</t> integrated <t>fluorescence</t> intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system (Sartorius). Statistical significance was assessed by two-way ANOVA. ( C ) Virus spread following infection with RSV virus stocks A-C in A549 cells infected at an MOI of 1, with medium replaced by Opti-MEM (medium B) after adsorption. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system. ( D ) Relative G protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( E ) Relative F protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant
    Incucyte Live Cell Analysis System, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pmc13621201-318-20-24
    Average 99 stars, based on 1 article reviews
    incucyte live cell analysis system - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG cell imaging
    Medium-dependent effects on RSV G protein size, relative protein signals in virus stocks, and viral spread. ( A ) Western blot analysis of soluble G (sG) protein derived from HEK293T cells (top), HEK293T GALE/GALK1 knockout cells (middle), and whole-cell lysates of RSV A-0594-EGFP-infected A549 cells (bottom, 24 hpi), all produced or infected in cell culture medium A, B or C. ( B ) Culture medium-dependent viral spread of RSV A-0594-EGFP in A549 cells infected at an MOI of 1 and cultured in media A, B, or C. <t>GFP</t> integrated <t>fluorescence</t> intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system (Sartorius). Statistical significance was assessed by two-way ANOVA. ( C ) Virus spread following infection with RSV virus stocks A-C in A549 cells infected at an MOI of 1, with medium replaced by Opti-MEM (medium B) after adsorption. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system. ( D ) Relative G protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( E ) Relative F protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant
    Cell Imaging, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pm42803585-95-8-19
    Average 99 stars, based on 1 article reviews
    cell imaging - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Sartorius AG resource source identifier incucyte software v2024a sartorius
    Medium-dependent effects on RSV G protein size, relative protein signals in virus stocks, and viral spread. ( A ) Western blot analysis of soluble G (sG) protein derived from HEK293T cells (top), HEK293T GALE/GALK1 knockout cells (middle), and whole-cell lysates of RSV A-0594-EGFP-infected A549 cells (bottom, 24 hpi), all produced or infected in cell culture medium A, B or C. ( B ) Culture medium-dependent viral spread of RSV A-0594-EGFP in A549 cells infected at an MOI of 1 and cultured in media A, B, or C. <t>GFP</t> integrated <t>fluorescence</t> intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system (Sartorius). Statistical significance was assessed by two-way ANOVA. ( C ) Virus spread following infection with RSV virus stocks A-C in A549 cells infected at an MOI of 1, with medium replaced by Opti-MEM (medium B) after adsorption. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system. ( D ) Relative G protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( E ) Relative F protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant
    Resource Source Identifier Incucyte Software V2024a Sartorius, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+cell+analysis+instruments/Live+Cell+Analysis+Instruments/pm42805174-820-2-8
    Average 99 stars, based on 1 article reviews
    resource source identifier incucyte software v2024a sartorius - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    ART6043 potentiates PARP inhibition in vitro . A, Representative CTG assay combination matrix for DLD-1 BRCA2 −/− (left) and DLD-1 wild-type (right) cells treated with titrated ART6043 and talazoparib for 14 days. Intensity of blue color indicates the level of synergy. % Control indicates relative viability. B, Representative IncuCyte growth curves for DLD-1 BRCA2 −/− cells treated with ART6043 or talazoparib as monotherapy or in combination at the indicated doses for 14 days. C, As for A but using olaparib instead of talazoparib. D, As for B but using olaparib instead of talazoparib. ART6043 concentrations represent free drug in tissue culture medium.

    Journal: Clinical Cancer Research

    Article Title: The Pharmacologic Profile of ART6043, a First-in-Class Clinical DNA Polymerase Theta Polymerase Domain Inhibitor Potentiating PARP Inhibitor Efficacy

    doi: 10.1158/1078-0432.CCR-26-0493

    Figure Lengend Snippet: ART6043 potentiates PARP inhibition in vitro . A, Representative CTG assay combination matrix for DLD-1 BRCA2 −/− (left) and DLD-1 wild-type (right) cells treated with titrated ART6043 and talazoparib for 14 days. Intensity of blue color indicates the level of synergy. % Control indicates relative viability. B, Representative IncuCyte growth curves for DLD-1 BRCA2 −/− cells treated with ART6043 or talazoparib as monotherapy or in combination at the indicated doses for 14 days. C, As for A but using olaparib instead of talazoparib. D, As for B but using olaparib instead of talazoparib. ART6043 concentrations represent free drug in tissue culture medium.

    Article Snippet: Cells were imaged on the IncuCyte Live-Cell Imaging System (Essen Biosciences, RRID:SCR_023147).

    Techniques: Inhibition, In Vitro, CTG Assay, Control, Tissue

    CLSP-1025 demonstrates functional activity in various cellular systems including cell cocultures and patient-derived organoids. A, CLSP-1025–mediated cytotoxicity in cocultures of PBMCs and HLA-A*02:01 + –p53 R175H+ tumor cells. Cytotoxicity is reported as the percent tumor-cell death after a 96-hour coculture (mean ± SEM; n = 2). B, HLA-A*02:01 surface expression on p53 R175H+ TYK-nu cells after 72 hours of IFN-γ treatment, measured by geometric mean fluorescence intensity (gMFI) via flow cytometry. Data shown as mean; n = 3 technical replicates. C, CLSP-1025–mediated cytotoxicity in PBMC cocultures with TYK-nu cells with or without IFN-γ pretreatment, shown as percent tumor-cell death (mean ± SEM; n = 3 technical replicates). D–E, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 R175H + adenocarcinoma organoids, measured by percent tumor-cell death and MSD, respectively [mean ± standard deviation (SD); n = 1 PBMC donor and technical duplicates for IFN-γ measurements]. F and G, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 WT adenocarcinoma organoids. H, CLSP-1025–mediated killing of GFP + KLE cells when cocultured with isolated CD4 + , CD8 + , or bulk CD3 + T cells. GFP signal was quantified in real time using Incucyte and plotted as normalized fluorescence units [GFP fluorescence units (GFU) × μm 2 /image]. Data shown as mean ± SD; n = 2 technical replicates.

    Journal: Clinical Cancer Research

    Article Title: Preclinical Characterization of CLSP-1025, a First-in-Class, Mutation-Specific T-Cell Engager Targeting a Neoantigen Derived from a Common p53 Mutation

    doi: 10.1158/1078-0432.CCR-26-1252

    Figure Lengend Snippet: CLSP-1025 demonstrates functional activity in various cellular systems including cell cocultures and patient-derived organoids. A, CLSP-1025–mediated cytotoxicity in cocultures of PBMCs and HLA-A*02:01 + –p53 R175H+ tumor cells. Cytotoxicity is reported as the percent tumor-cell death after a 96-hour coculture (mean ± SEM; n = 2). B, HLA-A*02:01 surface expression on p53 R175H+ TYK-nu cells after 72 hours of IFN-γ treatment, measured by geometric mean fluorescence intensity (gMFI) via flow cytometry. Data shown as mean; n = 3 technical replicates. C, CLSP-1025–mediated cytotoxicity in PBMC cocultures with TYK-nu cells with or without IFN-γ pretreatment, shown as percent tumor-cell death (mean ± SEM; n = 3 technical replicates). D–E, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 R175H + adenocarcinoma organoids, measured by percent tumor-cell death and MSD, respectively [mean ± standard deviation (SD); n = 1 PBMC donor and technical duplicates for IFN-γ measurements]. F and G, CLSP-1025–mediated cytotoxicity and IFN-γ secretion in PBMC cocultures with HLA-A*02:01 + –p53 WT adenocarcinoma organoids. H, CLSP-1025–mediated killing of GFP + KLE cells when cocultured with isolated CD4 + , CD8 + , or bulk CD3 + T cells. GFP signal was quantified in real time using Incucyte and plotted as normalized fluorescence units [GFP fluorescence units (GFU) × μm 2 /image]. Data shown as mean ± SD; n = 2 technical replicates.

    Article Snippet: The plates were incubated in an Incucyte S3 imager (Sartorius: Incucyte S3 live-cell analysis system, RRID: SCR_023147) for 160 hours and whole-well images captured every 4 hours.

    Techniques: Functional Assay, Activity Assay, Patient-Derived Organoids, Expressing, Fluorescence, Flow Cytometry, Organoids, Standard Deviation, Isolation

    Medium-dependent effects on RSV G protein size, relative protein signals in virus stocks, and viral spread. ( A ) Western blot analysis of soluble G (sG) protein derived from HEK293T cells (top), HEK293T GALE/GALK1 knockout cells (middle), and whole-cell lysates of RSV A-0594-EGFP-infected A549 cells (bottom, 24 hpi), all produced or infected in cell culture medium A, B or C. ( B ) Culture medium-dependent viral spread of RSV A-0594-EGFP in A549 cells infected at an MOI of 1 and cultured in media A, B, or C. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system (Sartorius). Statistical significance was assessed by two-way ANOVA. ( C ) Virus spread following infection with RSV virus stocks A-C in A549 cells infected at an MOI of 1, with medium replaced by Opti-MEM (medium B) after adsorption. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system. ( D ) Relative G protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( E ) Relative F protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant

    Journal: Virology Journal

    Article Title: Cell culture medium influences RSV G protein glycosylation and recognition by monoclonal antibodies

    doi: 10.1186/s12985-026-03318-8

    Figure Lengend Snippet: Medium-dependent effects on RSV G protein size, relative protein signals in virus stocks, and viral spread. ( A ) Western blot analysis of soluble G (sG) protein derived from HEK293T cells (top), HEK293T GALE/GALK1 knockout cells (middle), and whole-cell lysates of RSV A-0594-EGFP-infected A549 cells (bottom, 24 hpi), all produced or infected in cell culture medium A, B or C. ( B ) Culture medium-dependent viral spread of RSV A-0594-EGFP in A549 cells infected at an MOI of 1 and cultured in media A, B, or C. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system (Sartorius). Statistical significance was assessed by two-way ANOVA. ( C ) Virus spread following infection with RSV virus stocks A-C in A549 cells infected at an MOI of 1, with medium replaced by Opti-MEM (medium B) after adsorption. GFP integrated fluorescence intensity (GCU × µm²/image; mean ± SD, n = 9 from three independent experiments) was quantified over time using an IncuCyte system. ( D ) Relative G protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( E ) Relative F protein signal normalised to N protein signal in lysed RSV A-0594-EGFP virus stocks A-C, determined by Western blot densitometry (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant

    Article Snippet: Integrated GFP fluorescence (GCU × μm2/image) was quantified over 5 days using an IncuCyte S3 system (Sartorius).

    Techniques: Virus, Western Blot, Analysis, Derivative Assay, Knock-Out, Infection, Produced, Cell Culture, Fluorescence, Adsorption

    Antibody binding and neutralization of recombinant RSV produced in medium A, B or C. ( A ) Quantification of red (therapeutic F-specific antibodies: palivizumab, which binds both the pre-F and post-F conformations, and the pre-F-specific antibodies nirsevimab and clesrovimab) and blue (post-F-specific control antibody MAB8599) fluorescence intensity, normalized to GFP signal, for infected cells cultured in medium A, B or C (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( B ) Representative fluorescence images of infected cells stained with palivizumab, nirsevimab and clesrovimab (red) and the post-F control antibody (blue). ( C ) IC50 values of palivizumab, nirsevimab and clesrovimab, against virus produced with medium B or C, determined by 4-PL regression (mean ± SD, n = 3 independent experiments). Statistical significance was assessed using fitted IC50 values. ( D ) Scatter plot showing RT-qPCR Cq values versus TCID50 values for virus stocks A-C. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant

    Journal: Virology Journal

    Article Title: Cell culture medium influences RSV G protein glycosylation and recognition by monoclonal antibodies

    doi: 10.1186/s12985-026-03318-8

    Figure Lengend Snippet: Antibody binding and neutralization of recombinant RSV produced in medium A, B or C. ( A ) Quantification of red (therapeutic F-specific antibodies: palivizumab, which binds both the pre-F and post-F conformations, and the pre-F-specific antibodies nirsevimab and clesrovimab) and blue (post-F-specific control antibody MAB8599) fluorescence intensity, normalized to GFP signal, for infected cells cultured in medium A, B or C (mean ± SD, n = 3). Statistical significance was assessed by one-way ANOVA. ( B ) Representative fluorescence images of infected cells stained with palivizumab, nirsevimab and clesrovimab (red) and the post-F control antibody (blue). ( C ) IC50 values of palivizumab, nirsevimab and clesrovimab, against virus produced with medium B or C, determined by 4-PL regression (mean ± SD, n = 3 independent experiments). Statistical significance was assessed using fitted IC50 values. ( D ) Scatter plot showing RT-qPCR Cq values versus TCID50 values for virus stocks A-C. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. ns, not significant

    Article Snippet: Integrated GFP fluorescence (GCU × μm2/image) was quantified over 5 days using an IncuCyte S3 system (Sartorius).

    Techniques: Binding Assay, Neutralization, Recombinant, Produced, Control, Fluorescence, Infection, Cell Culture, Staining, Virus, Quantitative RT-PCR